All predesigns are based on the most recent public genome sequencing data available and will be updated regularly.
Sigma CRISPR predesigns are created with the goal of minimizing or eliminating off-targeting. All Sigma CRISPR targets are unique within the genome and must meet additional stringent design criteria.
If your goal is to target a specific isoform of your gene, search by RefSeq number instead of gene name in the CRISPR design tool.
If your gene name is not producing results, search for it using the RefSeq ID; some genes have many common names and not all may be recognized by the design tool.
If you do not see any CRISPR designs for your gene name or RefSeq number, please contact us for a custom design by following the custom order form link.
We recommend screening 4 Sigma CRISPRs for each gene target to find the most active nuclease.
For knockins, it is advisable to identify the best Sigma CRISPR for your region of interest BEFORE designing donor constructs.
For knocking out a gene, we recommend targeting an exon within the first 2/3 of the gene's coding region.
